上海易色医疗科技有限公司
Shanghai Yise Medical Technology Co.,Ltd.
杂志：Stem Cell Rev. 2016 Feb;12(1):156-61. doi:
10.1007/s12015-015-9630-8.
标题：Mycoplasma Infection Alters Cancer Stem Cell Properties
in Vitro.
作者：Gedye C1,2, Cardwell T1,3, Dimopoulos N1, Tan BS1, Jackson H1, Svobodová S1, Anaka M1, Behren A1,3, Maher C4,5, Hofmann O4,6, Hide W4,6,Caballero O7, Davis ID1,8, Cebon J9,10.
英文摘要：Cancer cell lines can be useful to model cancer
stem cells. Infection with Mycoplasma species is an insidious problem
in mammalian cell culture. While investigating stem-like properties in
early passage melanoma cell lines, we noted poorly reproducible results
from an aliquot of a cell line that was later found to be infected
with Mycoplasma hyorhinis. Deliberate infection of other early
passage melanoma cell lines aliquots induced variable and
unpredictable effects on expression of putative cancer stem cell markers,
clonogenicity, proliferation and global gene expression. Cell lines
established in stem cell media (SCM) were equally susceptible. Mycoplasma status
is rarely reported in publications using cultured cells to study the cancer
stemcell hypothesis. Our work highlights the importance of surveillance
for Mycoplasma infection while using any cultured cells to
interrogate tumor heterogeneity.