上海易色医疗科技有限公司
Shanghai Yise Medical Technology Co.,Ltd.
杂志：Anal Bioanal Chem. 2016 Jun;408(16):4267-73. doi:
10.1007/s00216-016-9525-9. Epub 2016 Apr 13.
标题：Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells.
作者：Yu T1, Wang Y1, Zhang H1, Johnson CH2, Jiang Y1, Li X3, Wu Z3, Liu T3, Krausz KW4, Yu A5, Gonzalez FJ4, Huang M1, Bi H6.
英文摘要：Mycoplasma contamination is a common problem in cell
culture and can alter cellular functions. Since cell metabolism
is either directly or indirectly involved in every aspect of cell function,
it is important to detect changes to the cellular metabolome after mycoplasma infection.
In this study, liquid chromatography mass spectrometry (LC/MS)-based
metabolomics was used to investigate the effect of mycoplasma contamination
on the cellular metabolism of human pancreatic carcinoma cells (PANC-1).
Multivariate analysis demonstrated that mycoplasma contamination
induced significant metabolic changes in PANC-1 cells. Twenty-three metabolites
were identified and found to be involved in arginine and purine metabolism and
energy supply. This study demonstrates that mycoplasma contamination
significantly alters cellular metabolite levels, confirming the compelling need
for routine checking of cell cultures for mycoplasma contamination,
particularly when used for metabolomics studies. Graphical abstract
Metabolomics reveals mycoplasma contamination changes the metabolome
of PANC-1 cells.
中文摘要：支原体污染是细胞培养中的一个常见问题，并可以改变细胞的功能。由于细胞代谢直接或间接参与细胞功能的各个方面，它的变化来检测细胞代谢组支原体感染后重要。在这项研究中，液相色谱-质谱（LC / MS）为基础的代谢组学方法研究了支原体污染对人胰腺癌细胞的细胞代谢的影响（株）。多因素分析表明，支原体污染诱导PANC-1细胞显著的代谢变化。二零三个代谢产物被确定，并发现参与精氨酸和嘌呤代谢和能源供应。这项研究表明，支原体污染明显改变细胞的代谢水平，确定为支原体污染的细胞培养常规检查的迫切需要，尤其是当用于代谢组学研究。图形抽象代谢组学揭示支原体污染PANC-1细胞代谢的变化。